Ouchterlony Double Diffusion PDF Download: A Low-Tech Procedure for Antigen-Antibody Interactions

Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens. The technique is named after Örjan Ouchterlony, the Swedish physician who developed the test in 1948 to evaluate the production diphtheria toxins from isolated bacteria.[1]

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It is more commonly known as Ouchterlony double diffusion or passive double immunodiffusion. In this method, both the antigen and antibody diffuse independently through agar gel in two dimensions, horizontally and vertically.

In the gel double-diffusion test (Ouchterlony test), the virus-antibody reaction takes place in agar gel. Virus and antiserum are placed in opposite wells in an agar plate. Virus particles and antibodies then diffuse into the agar. If the virus coat proteins possess antigenic determinants for which there are matching antibodies in the serum, a precipitate is formed where virus and antibodies meet in optimal proportions. The precipitate is visible in the agar as a white line between the wells. 2ff7e9595c